Effects of timosaponins on learning and memory abilities of rats with dementia induced by lateral cerebral ventricular injection of amyloid β-peptide


BIBLIOGRAPHIC THERAPEUTIC AGENT ANIMAL MODEL EXPERIMENTAL DESIGN OUTCOMES

Bibliographic

Year of Publication:
2005
Contact PI Name:
Shi Ouyang
Contact PI Affiliation:
Department of Pharmacology, Southern Medical University, Guangzhou, China
Co-Authors:
Li-sha Sun, Sheng-lan Guo, Xu Liu, Jiang-ping Xu
Primary Reference (PubMED ID):
Funding Source:
National 863 Program of China for New Drug Research and Development
Natural Science Foundation of Guangdong Province
Study Goal and Principal Findings:

Objective: To investigate the effects of timosaponins, one group of the two major components of Anemarrhean asphodeloides Bge, on the learning and memory capacities of rats with dementia induced by amyloid beta-peptide (25-35) [Abeta (25-35)].

Methods: Sixty SD rats were randomized into 6 groups (n=10) and except for those in the control group, all other rats were subjected to lateral cerebral ventriclar injection of aggregated Abeta (25-35) to prepare rat models of dementia. Twenty- four hours after the injection, the rats received intragastric administration of timosaponins at 3 different doses (treatment group) or Ginkgo biloba extract EGB761 on a daily basis for 14 consecutive days. From postoperative days 8 to 14 after Abeta (25-35) injection, Morris water maze test was performed to evaluate the effects of Abeta (25-35) and the therapeutic agents timosaponins on the learning and memory capacity of the rats. On day 14, the level of malonaldehyde (MDA), superoxide dismutase (SOD) activity and total antioxidation capacity in the brain tissue of the rats were measured.

Results: Abeta (25-35) induced significant learning and memory impairment in the rats, which had lowered SOD activity and total antioxidation capacity (P<0.01) with elevated MDA level (P<0.05). Compared with the rats in dementia model group, those receiving timosaponin treatment at different doses all manifested alleviation of learning and memory impairment (P<0.05), with enhanced SOD activity (P<0.05) and total antioxidation capacity (P<0.01) and reduced MDA level (P<0.05) in the brain tissue.

Conclusion: Timosaponins can remarkably enhance the learning and memory capacities in rats with Abeta (25-35)-induced dementia, presumably in relation to their actions to promote the scavenging of the free radicals.

Therapeutic Agent

Therapeutic Information:
Therapy Type:
Natural Product
Therapeutic Agent:
Timosaponin
Therapeutic Target:
Multi Target
Therapy Type:
Natural Product
Therapeutic Agent:
Ginkgo Biloba Extract (EGb761)
Therapeutic Target:
Multi Target

Animal Model

Model Information:
Species:
Rat
Model Type:
beta Amyloid Peptide Injection
Strain/Genetic Background:
Not Applicable

Experimental Design

Is the following information reported in the study?:
Power/Sample Size Calculation
Randomized into Groups
Blinded for Treatment
Blinded for Outcome Measures
Pharmacokinetic Measures
Pharmacodynamic Measures
Toxicology Measures
ADME Measures
Biomarkers
Dose
Formulation
Route of Delivery
Duration of Treatment
Frequency of Administration
Age of Animal at the Beginning of Treatment
Age of Animal at the End of Treatment
Sex as a Biological Variable
Study Balanced for Sex as a Biological Variable
Number of Premature Deaths
Number of Excluded Animals
Statistical Plan
Genetic Background
Inclusion/Exclusion Criteria Included
Conflict of Interest
Experiment Notes

Age of Animal: In studies using rats, typically the rat weight is reported rather than age. A male Sprague Dawley rat weighing 200-250g is between 6-8 weeks old.

Outcomes

Outcome Measured
Outcome Parameters
Behavioral
Morris Water Maze
Biochemical
Malondialdehyde (MDA)
Superoxide Dismutase (SOD)
Superoxide Dismutase (SOD) Activity
Total Antioxidant Capacity (TAC)

Source URL: http://alzped.nia.nih.gov/effects-timosaponins-learning